Journal: Lipids in Health and Disease

Article Title: Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities

doi: 10.1186/s12944-019-1114-4

Figure Lengend Snippet: Body weight change, fat pad weight, liver weight, and hepatic triglyceride (TG) and malondialdehyde (MDA) levels in mice fed a methionine-choline-deficient (MCD) or control diet and housed in two different animal facilities (WT1, WT2). a Body weight (BW) at the end of the study relative to the initial weight in %. b Epididymal (Epi) fat pad weight normalized to BW. c Liver weight normalized to BW. d H&E stained liver of WT mice fed a control diet. Small lipid droplets appear in the liver of WT2 mice and are visible at higher magnification (second row). Large lipid droplets in the liver of MCD diet fed mice (third and fourth row). e Oil Red O stained liver. f Hepatic TG concentrations. g Hepatic MDA levels. Data of 5 to 8 animals per group are shown. * p < 0.05, ** p < 0.01

Article Snippet: Fifteen mice (14 weeks old - 8 WT1 and 7 WT2) were fed the MCD diet (E15653–94, Ssniff, Soest, Germany) and eleven mice (14 weeks old - 6 WT1 and 5 WT2 animals) the respective control chow (E15654–04, Ssniff, Soest, Germany) for 2 weeks.

Techniques: Control, Staining

Journal: Lipids in Health and Disease

Article Title: Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities

doi: 10.1186/s12944-019-1114-4

Figure Lengend Snippet: Hepatic expression of inflammatory and profibrotic genes in mice fed a methionine-choline-deficient (MCD) or control diet and housed in two different animal facilities (WT1, WT2). a Hepatic F4/80 mRNA. b Hepatic CD68 mRNA. c Hepatic TNF mRNA. d Hepatic IL-6 mRNA. e Hepatic chemerin mRNA. f Hepatic chemerin protein. g Quantification of hepatic chemerin protein. h Hepatic TGF beta mRNA. i Hepatic alpha SMA mRNA. Data of 5 to 8 animals per group are shown. * p < 0.05, ** p < 0.01

Article Snippet: Fifteen mice (14 weeks old - 8 WT1 and 7 WT2) were fed the MCD diet (E15653–94, Ssniff, Soest, Germany) and eleven mice (14 weeks old - 6 WT1 and 5 WT2 animals) the respective control chow (E15654–04, Ssniff, Soest, Germany) for 2 weeks.

Techniques: Expressing, Control

Journal: Lipids in Health and Disease

Article Title: Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities

doi: 10.1186/s12944-019-1114-4

Figure Lengend Snippet: Cholesteryl ester (CE), free cholesterol (FC), sphingomyelin (SM) and ceramide (Cer) in the liver of mice fed a methionine-choline-deficient (MCD) or control diet and housed in two different animal facilities (WT1, WT2). Concentrations are given as nmol/mg wet weight. a CE b FC c CE 22:6 d Total SM e SM 40:1 f SM 34:1 g Total Cer h Cer d18:1/16:0 and ( i ) Cer d18:1/22:0. Data of 5 to 8 animals per group are shown. * p < 0.05, ** p < 0.01

Article Snippet: Fifteen mice (14 weeks old - 8 WT1 and 7 WT2) were fed the MCD diet (E15653–94, Ssniff, Soest, Germany) and eleven mice (14 weeks old - 6 WT1 and 5 WT2 animals) the respective control chow (E15654–04, Ssniff, Soest, Germany) for 2 weeks.

Techniques: Control

Journal: Lipids in Health and Disease

Article Title: Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities

doi: 10.1186/s12944-019-1114-4

Figure Lengend Snippet: Lysophosphatidylcholine (LPC), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) in the liver of mice fed a methionine-choline-deficient (MCD) or control diet and housed in two different animal facilities (WT1, WT2). Concentrations are given as nmol/mg wet weight. a LPC 20:3 b LPC 16:0 c saturated (Sat) LPC d monounsaturated (MUFA) PC e PC 32:1 f PC 30:0 g PC to PE ratio h PE 36:5 and i PE 40:6. Data of 5 to 8 animals per group are shown. * p < 0.05, ** p < 0.01

Article Snippet: Fifteen mice (14 weeks old - 8 WT1 and 7 WT2) were fed the MCD diet (E15653–94, Ssniff, Soest, Germany) and eleven mice (14 weeks old - 6 WT1 and 5 WT2 animals) the respective control chow (E15654–04, Ssniff, Soest, Germany) for 2 weeks.

Techniques: Control

Journal: Lipids in Health and Disease

Article Title: Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities

doi: 10.1186/s12944-019-1114-4

Figure Lengend Snippet: Phosphatidylserine (PS) and phosphatidylinositol (PI) in the liver of mice fed a methionine-choline-deficient (MCD) or control diet and housed in two different animal facilities (WT1, WT2). Concentrations are given as nmol/mg wet weight. a PS 40:5 b Monounsaturated (MUFA) PS c PS 36:4 d PI 40:4 e PI 34:2 and f PI 40:5. Data of 5 to 8 animals per group are shown. * p < 0.05, ** p < 0.01

Article Snippet: Fifteen mice (14 weeks old - 8 WT1 and 7 WT2) were fed the MCD diet (E15653–94, Ssniff, Soest, Germany) and eleven mice (14 weeks old - 6 WT1 and 5 WT2 animals) the respective control chow (E15654–04, Ssniff, Soest, Germany) for 2 weeks.

Techniques: Control

Journal: Toxicological Research

Article Title: Evaluating the Influence of Side Stream Cigarette Smoke at an Early Stage of Non-Alcoholic Steatohepatitis Progression in Mice

doi: 10.5487/TR.2017.33.1.031

Figure Lengend Snippet: Weight gain and liver injury caused by exposure to SSCS. Six-week-old male mice were divided control and MCDHF diet group. Each group was divided into three groups: non-exposed mice (NE), mice exposed to low dose of SSCS (CS 20), high-dose-exposed mice (CS 40). Five mice were used per group. (A) Changes in body weight, liver weight and liver to body weight ratio for 6 weeks of the experiment. (B) The serum AST and ALT levels were presented among the control and MCDHF diet feeding groups with or without SSCS exposure. Values are expressed as the mean ± SEM. Experimental groups marked by different letters represent significant differences between groups at p <0.05.

Article Snippet: The mice were housed in a standardized (24 ± 2°C, 50 ± 5% humidity), pathogen-free environment and fed a sterile standard control diet or a MCDHF diet (Research diet Inc., New Brunswick, NJ, USA).

Techniques: Control

Journal: Toxicological Research

Article Title: Evaluating the Influence of Side Stream Cigarette Smoke at an Early Stage of Non-Alcoholic Steatohepatitis Progression in Mice

doi: 10.5487/TR.2017.33.1.031

Figure Lengend Snippet: Histopathological findings in liver. (A) Representative H&E-stained liver sections are shown. Pictures are shown at 200× magnification. (B) NAS for mouse liver specimens of experimental groups; Steatosis, inflammation, and hepatocyte ballooning degeneration were categorized, and the sum of these scores was designated as NAS. Steatosis : Gr.0: < 5%, Gr.1: 5~33%, Gr.2: > 33~66%, Gr.3: > 66%, Lobular inflammation : Gr.0: No foci, Gr.1: < 2 foci, Gr.2: 2~4 foci, Gr.3: > 4 foci per X200 field, Ballooning degeneration: Gr.0: < 5%, Gr.1: > 5~33%, Gr.2: > 33~66%, Gr.3: > 66%, NAS: Score 0~2: not NASH, 3~4: borderline, 5~8: NASH (total score, Control diet group - NE : 0.4, CS20: 0.8, CS40: 0.6, MCDHF diet group - NE: 5.4, CS20: 5.6, CS40: 5.6).

Article Snippet: The mice were housed in a standardized (24 ± 2°C, 50 ± 5% humidity), pathogen-free environment and fed a sterile standard control diet or a MCDHF diet (Research diet Inc., New Brunswick, NJ, USA).

Techniques: Staining, Control

Journal: Toxicological Research

Article Title: Evaluating the Influence of Side Stream Cigarette Smoke at an Early Stage of Non-Alcoholic Steatohepatitis Progression in Mice

doi: 10.5487/TR.2017.33.1.031

Figure Lengend Snippet: Evaluation of lipid accumulation in liver. (A) Hepatic TC and TG content were measured using commercial kit. (B) Sections of liver tissue from control and MCDHF diet group with or without exposure to SSCS. Tissues were fixed and sections were stained with Oil Red O to observe the hepatic steatosis. Pictures are shown at 200× magnification. (C) Lipid accumulation was evaluated by using an image analysis system. Values are expressed as the mean ± SEM. Experimental groups marked by different letters represent significant differences between groups at p <0.05.

Article Snippet: The mice were housed in a standardized (24 ± 2°C, 50 ± 5% humidity), pathogen-free environment and fed a sterile standard control diet or a MCDHF diet (Research diet Inc., New Brunswick, NJ, USA).

Techniques: Control, Staining

Journal: Toxicological Research

Article Title: Evaluating the Influence of Side Stream Cigarette Smoke at an Early Stage of Non-Alcoholic Steatohepatitis Progression in Mice

doi: 10.5487/TR.2017.33.1.031

Figure Lengend Snippet: Additional experiments with increasing SSCS concentration and exposure time. Mice were divided into three groups: non-exposed mice (NE), mice exposed to low dose of SSCS (CS 30), high-dose-exposed mice (CS 60). (A) Effects of SSCS exposure on body weight, liver weight and liver to body weight ratio for 6 weeks of the experiment. (B) Representative H&E-stained liver sections are shown. Pictures are shown at 200× magnification. (C) Histological analysis of H&E-stained liver sections using NAS. (D) The serum AST, ALT, hepatic TC and TG levels were presented among the control and MCDHF diet feeding groups with or without SSCS exposure. Values are expressed as the mean ± SEM. Experimental groups marked by different letters represent significant differences between groups at p < 0.05.

Article Snippet: The mice were housed in a standardized (24 ± 2°C, 50 ± 5% humidity), pathogen-free environment and fed a sterile standard control diet or a MCDHF diet (Research diet Inc., New Brunswick, NJ, USA).

Techniques: Concentration Assay, Staining, Control

Journal: Journal of biochemical and molecular toxicology

Article Title: Shc inhibitor idebenone ameliorates liver injury and fibrosis in dietary NASH in mice

doi: 10.1002/jbt.22876

Figure Lengend Snippet: Shc expression and activation in PBMC and liver in MCD model. Six-week-old male C57B6J mice were fed with an MCD diet for 6 weeks with idebenone 10 or 40 mg/kg oral daily starting at Day 1 of the diet representing a preventive treatment. Shc phosphorylation and activation in PBMC (A) and liver (B) were analyzed with either Jess capillary Western blot or conventional Western blot analysis. The image signal was quantified with the Compass software. Phosphorylation and expression of p52Shc increased in PBMC in the MCD group and dramatically reduced by idebenone on both 10 and 40 mg/kg. In the liver, Shc expression had a trend of reduction by idebenone, but less extent compared to PBMC. Representative images are shown; mean ± SD, N = 4. Ctrl, control; Ide, idebenone; MCD, methionine-choline deficient; PBMC, peripheral blood mononuclear cell. *p < 0.05

Article Snippet: For the MCD model, the 8-week old mice were given either a control diet supplemented with methionine and choline or an MCD diet (Bio-Serv) for 6 weeks.

Techniques: Expressing, Activation Assay, Western Blot, Software

Journal: Journal of biochemical and molecular toxicology

Article Title: Shc inhibitor idebenone ameliorates liver injury and fibrosis in dietary NASH in mice

doi: 10.1002/jbt.22876

Figure Lengend Snippet: Idebenone protects mice from MCD diet-induced liver injury. The mice were on an MCD diet with preventive idebenone dosing for 6 weeks. Serological study showed that idebenone 40 mg/kg decreased the ALT level significantly (A). AST had a trend of decrease at both 10 and 40 mg/kg (B). Real-time qPCR on liver tissues (C) showed that the idebenone reduced the transcripts of fibrogenic genes (Col1a1, αSMA, TGFβ, MMP2, and Timp1) significantly. The reduction of Col1a1 showed a dose-dependent trend. Hydroxyproline assay (D) showed a lower amount of collagen content detected in both dosage groups. Picrosirius red staining and H&E (E) showed improved fibrosis and histology in the treated mice. Arrows indicate the patches of inflammatory cells; mean ± SD, N = 8–10. ALT, aminotransferase; AST, aspartate aminotransferase; Col1a1, collagen type I alpha 1 chain; H&E, hematoxylin and eosin; Ide, idebenone; MCD, methionine-choline deficient; MMP2, matrix metalloproteinase 2; qPCR, quantitative polymerase chain reaction; TGFβ, transforming growth factor-β; αSMA, α-smooth muscle actin. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: For the MCD model, the 8-week old mice were given either a control diet supplemented with methionine and choline or an MCD diet (Bio-Serv) for 6 weeks.

Techniques: Hydroxyproline Assay, Staining, Real-time Polymerase Chain Reaction